Mitochondrial topoisomerase II activity is essential for kinetoplast DNA minicircle segregation.
نویسندگان
چکیده
منابع مشابه
TbPIF1, a Trypanosoma brucei mitochondrial DNA helicase, is essential for kinetoplast minicircle replication.
Kinetoplast DNA, the trypanosome mitochondrial genome, is a network of interlocked DNA rings including several thousand minicircles and a few dozen maxicircles. Minicircles replicate after release from the network, and their progeny reattach. Remarkably, trypanosomes have six mitochondrial DNA helicases related to yeast PIF1 helicase. Here we report that one of the six, TbPIF1, functions in min...
متن کاملA second mitochondrial DNA primase is essential for cell growth and kinetoplast minicircle DNA replication in Trypanosoma brucei.
The mitochondrial DNA of trypanosomes contains two types of circular DNAs, minicircles and maxicircles. Both minicircles and maxicircles replicate from specific replication origins by unidirectional theta-type intermediates. Initiation of the minicircle leading strand and also that of at least the first Okazaki fragment involve RNA priming. The Trypanosoma brucei genome encodes two mitochondria...
متن کاملA new function of Trypanosoma brucei mitochondrial topoisomerase II is to maintain kinetoplast DNA network topology.
The mitochondrial genome of Trypanosoma brucei, called kinetoplast DNA, is a network of topologically interlocked DNA rings including several thousand minicircles and a few dozen maxicircles. Kinetoplast DNA synthesis involves release of minicircles from the network, replication of the free minicircles and reattachment of the progeny. Here we report a new function of the mitochondrial topoisome...
متن کاملStructure of Leishmania minicircle kinetoplast DNA classes.
In a recent paper (5), Noyes et al. discussed a nested-PCRbased approach for identification and analysis of Leishmania kinetoplast DNA (kDNA) minicircle classes using the analysis of minicircle variable-region (VR) restriction profiles. The kDNA of trypanosomatids consists of catenated minicircle (approximately 5 3 10 to 5 3 10 per associate) and maxicircle (20 to 50 per associate) molecules (8...
متن کاملStructure of Leishmania Minicircle Kinetoplast DNA Classes
In a recent paper (5), Noyes et al. discussed a nested-PCRbased approach for identification and analysis of Leishmania kinetoplast DNA (kDNA) minicircle classes using the analysis of minicircle variable-region (VR) restriction profiles. The kDNA of trypanosomatids consists of catenated minicircle (approximately 5 3 10 to 5 3 10 per associate) and maxicircle (20 to 50 per associate) molecules (8...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1994
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.14.6.3660